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The germination and growth profiles of maize seed plants as evidence of recovery of oil-impacted soils were poor in CSS1 (10%) with pronounced abnormal morphology when compared with the data obtained for EXPS (74%) and CSS2 (80%). However, by day 35, there was complete disappearance of all peaks including the pristane and phytane molecules in the former whereas in CSS1, there were no observable changes. The GC fingerprints of alkane fractions of the recovered oil reduced significantly by day 15 for EXPS with near-similar results of CSS1. The corresponding value for CSS1 was 98,106.1 to 52,110 mg/kg soil, amounting to 46.88% oil disappearance. In EXPS, the residual oil decreased from 98,045 to 1,102.3 mg/kg soil at day 35 representing about 98.88% degradation. Similarly, the population densities of microflora in the polluted soils showed an initial decrease between days 0 and 5 before assuming an increasing trend with percent hydrocarbon-utilizers ranging significantly (P<0.05) from 0.56 to 6.6, 0.1 to 2.46 and 0.56 to 0.26, respectively for EXPS, CSS1, and CSS2. In contrast, the properties of CSS2 remained relatively stable throughout the investigated period. There were dramatic changes in the physico-chemistry of systems EXPS and CSS1 with utilization of the inorganic nutrients to near-depletion in the former than the latter.
Similar polluted soil without CSL amendment (CSS1) and uncontaminated soil (CSS2) served as controls. Soil artificially contaminated with diesel oil, treated with cassava steep liquor (CSL) and designated EXPS.
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